Biomedical Instrumentation Center

Contact Information

BIC is located on the ground floor of B building in rooms G230 - G242.

Uniformed Services University

Microscopic Imaging: Instruments

Zeiss 710 Confocal Laser Scanning Microscope

Zeiss 710The Zeiss 710 confocal laser scanning microscope consists of a Zeiss AxioImager inverted microscope equipped with four visible lasers as well as a tunable infrared laser for multi-photon excitation. This wide selection of excitation wavelengths coupled with user selectable emission wavelength ranges means that researchers are free to use almost any fluorescent molecule when designing their experiments. The system configuration allows researchers to collect images of up to 3 fluorescent channels and a transmitted light image simultaneously. The multi-photon excitation provided by the IR laser is ideal for deep imaging within tissues and also for FRET or uncaging studies in which the researcher needs to ensure that excitation and activation is limited to a single focal plane. The Zeiss 710 is also equipped with an environmental chamber which allows for the control of temperature, humidity and CO2 levels, making long-term live cell imaging possible.

Zeiss 700 Confocal Laser Scanning Microscope

Zeiss 700 Confocal Laser Scanning MicroscopeThe Zeiss 700 confocal laser scanning microscope consists of a Zeiss AxioObserver inverted microscope equipped with four lasers (405, 488, 561, and 633nm) to enable researchers to easily localize four distinct fluorescent signals (blue, green, red, and far-red). The system configuration allows researchers to collect images of up to 2 fluorescent channels and a transmitted light image simultaneously.

 

 

Multi-photon Confocal Electrophysiology System

Multi-photon Confocal Electrophysiology SystemThe multi-photon confocal electrophysiology system consists of a Zeiss AxioExaminer.Z1 upright microscope equipped with a direct-coupled Coherent Chameleon tunable infrared laser. The combination of the infrared laser, non-descanned detectors (currently green and red emission detection), and a range of long working distance water immersion objective lenses (10, 20 and 40x) allows for the deepest imaging possible in tissue slices or whole animal studies. The system is equipped with an Axon Multiclamp 700B amplifier and two headstages, mounted on motorized micromanipulators (Scientifica). A GRASS S88 dual output square pulse stimulator, a nano injector, and recording chamber perfusion and temperature control are also available. Electrophysiology components provide measurements of electrical activity and the ability for direct electrical stimulation in the tissue under 2-photon observation.

Zeiss Elyra.PS1

Zeiss Elyra.PS1The Zeiss Elyra.PS1 provides imaging modes and computational techniques that allow researchers to move their fluorescent experiments beyond the limit of resolution of conventional light microscopy. This system has four fluorescent channels (blue, green, red, and far-red) and operates in two different modes. In structured illumination (SIM) mode, images have approximately twice the lateral and axial resolution of conventional fluorescence microscopy while still being able to use standard fluorescent labels and specimen mounting techniques. In PALM or dSTORM mode, images may have up to 10 times the resolution of conventional fluorescent imaging (as small as 20nm in the X and Y dimensions and 40nm in the z dimension). This ultra-resolution mode requires special sample preparation techniques and selection of suitable fluorescent tags.

Zeiss Axioscan.Z1

Zeiss Axioscan.Z1The Zeiss Axioscan.Z1 is a complete solution for digitizing slide mounted samples in brightfield or 4-channel fluorescence. Up to 100 slides can be loaded and scanned at 5x, 10x, 20x, or 40x. The resulting images can be left in the native CZI format for analysis using Zeiss Zen software, or exported in other popular image formats such as TIFF or JPG.

 

 

Leica AF6000 Time-lapse Imaging System

The Leica AF6000 consists of a Leica inverted microscope equipped with a fully motorized 3-axis stage and CO2 and temperature control. Combined with the Leica AFS software package, the AF6000 is ideally suited for general fluorescence or transmitted light imaging. Potential applications include: (1) fluorescence imaging in blue, green, red, and far-red of cells or tissues in plates or on glass slides; (2) time-lapse imaging of live cells with fluorescence, transmitted light (brightfield or DIC), or both. The Leica Automatic Focus Control as well as software-based autofocus routines ensures that the specimen remains in focus during long experiments; (3)image tiling. The Leica AF6000 can collect multiple overlapping images of a large specimen and then stitch the images together to create a single image that provides both wide-field coverage and the ability to zoom in and see fine details; (4) deconvolution is an image processing method in which out of focus light which degrades image quality and resolution is removed. The integrated deconvolution package makes optimizing your images easy.

Stereology System

Stereology SystemThe stereology system is composed of a Zeiss AxioImager.M2 upright microscope and MicroBrightField's Stereo Investigator software package. The microscope is equipped with a wide range of objective lenses (2.5x - 63x), standard and oil immersion condensers, an X-Cite fluorescent light source, and filter cubes appropriate for imaging red, green, and blue fluorophores. The system also has a motorized stage and two digital cameras. The Zeiss Axiocam MRc is a color camera suitable for brightfield imaging, while the Hamamatsu Orca-R2 captures monochrome fluorescence images.

The Stereo Investigator software gives investigators image analysis, mapping, and measurement tools all in one software package, and allows for unbiased quantification of such parameters as cell numbers, lengths, areas, and volumes. All formulas and references used for calculations are provided with the software so that researchers are aware of exactly how the quantification is performed. The software also implements several statistical analysis methods so that users may have confidence in their results. More details about the Stereo Investigator software package can be found at:http://www.mbfbioscience.com/stereo-investigator

Leica UC6 ultramicrotome with FC6 cryo attachment

Leica UC6 ultramicrotome with FC6 cryo attachmentThe Leica UC6 is a state of the art ultramicrotome allowing users to obtain high quality sections (50 nanometers - 1 micrometer thick) of properly embedded specimens. The cryo attachment uses liquid nitrogen to keep knives and specimens at ultra-cold temperatures so that unembedded frozen material may be sectioned.

 

JEOL JEM-1011

JEOL-JEM1011The JEOL-JEM1011 transmission electron microscope allows for ultrastructural imaging of cells and tissues at magnifications up to and exceeding 100,000X. Crucial for experiments in which higher resolutions than those available through light microscopy are required. Cells and tissues can be sectioned and examined, or whole protein complexes, viral particles, etc. can be imaged through negative staining.

 

 

 

 

 

Multi-photon Confocal Electrophysiology System

Multi-photon Confocal Electrophysiology System

The multi-photon confocal electrophysiology system consists of a Zeiss AxioExaminer.Z1 upright microscope equipped with a direct-coupled Coherent Chameleon tunable infrared laser. The combination of the infrared laser, non-descanned detectors (blue, green and red emission detection), and a range of water immersion objective lenses (10, 20 and 40x) allows for the deepest imaging possible in tissue slices or in-vivo studies. Specialized objectives with extra-long working distance for cleared-tissue approaches are also available. The system is equipped with an Axon Multiclamp 700B amplifier and two headstages, mounted on motorized micromanipulators (Scientifica) and aGRASS S88 dual output square pulse stimulator. Additionally, optogenetics equipment is available, including a LED light source and assorted light guides and quartz fibers, to manipulate channelrhodopsin- or Halorhodopsin-expressing cells.  Electrophysiology components provide measurements of electrical activity and the ability for direct electrical stimulation in the tissue under 2-photon observation.