BIO
BIO - Local/NCA Faculty
Rachel T. Cox, Ph.D.
Name: Rachel T. Cox, Ph.D.
USU Department of Primary Appointment:
Biochemistry
Title:
Associate Professor (Tenured)
Faculty Rank:
Associate Professor
Location:
Uniformed Services University of the Health Sciences, Bethesda, MD
Research Interests:
Mechanisms governing mitochondrial function in vivo
Email:
Office Phone:
(301) 295-9791
Education
B.A. in Biology, University of Pennsylvania, Philadelphia, PA
Ph.D. in Genetics and Molecular Biology, University of North Carolina - Chapel Hill, Chapel Hill, NC
Postdoctoral Fellow, Department of Embryology, Carnegie Institute for Science, Baltimore, MD
Ph.D. in Genetics and Molecular Biology, University of North Carolina - Chapel Hill, Chapel Hill, NC
Postdoctoral Fellow, Department of Embryology, Carnegie Institute for Science, Baltimore, MD
Biography
Dr. Cox obtained her Ph.D. from the Curriculum in Genetics and Molecular Biology at University of North Carolina – Chapel Hill in 1992. She carried out her thesis work in the laboratory of Dr. Mark Peifer where she used the model organism Drosophila melanogaster to uncover how the highly conserved protein Armadillo/beta-catenin functions in cell-cell adhesion and cell signaling during development. After a year as a postdoctoral fellow in the Peifer lab, she went on to do a postdoctoral fellowship in the laboratory of Dr. Alan Spradling in the Department of Embryology, Carnegie Institute for Science in Baltimore, MD. There, she began studying the molecular mechanisms governing mitochondrial movement and function during Drosophila oogenesis. In 2008, Dr. Cox obtained an independent tenure track position at Uniformed Services University in the Department of Biochemistry and Molecular Biology where she continues her research on mitochondrial function and quality control during development and disease.
Representative publications, projects, and/or deployments
- Helen Hay Whitney Postdoctoral Research Fellowship, 1999-2002
- Hébert School of Medicine Faculty Impact Award, 2015
- Hébert School of Medicine Faculty Impact Award, 2017
- Outstanding Biomedical Educator Award, 2018
Bibliography
- Saoji, M., and Cox, R. T., Mitochondrial RNase P complex in animals: mt:tRNA processing and links to disease. In RNA Metabolism in Mitochondria; Cruz-Reyes, J. and Gray, M. W., Editors; Springer-Verlag Berlin Heidelberg: Berlin, Germany, 2018; in press.
- Sen, A. and Cox, R. T., Fly Models of Human Diseases: Drosophila as a Model for Understanding Human Mitochondrial Mutations and Disease. In: Leslie Pick, editor, Current Topics in Developmental Biology, Vol. 121, Burlington: Academic Press, 2017, pp. 1-27
- Sen, A., Karasik, A., Shanmuganathan, A., Mirkovic, E., Koutmos, M., and Cox, R.T. (2016) Loss of the mitochondrial protein-only Ribonuclease P complex causes aberrant tRNA processing and lethality in Drosophila. Nucleic Acids Research 44(13):6409-6422
- Sen, A. and Cox R. T. (2016) Clueless is a conserved ribonucleoprotein that binds the ribosome at the mitochondrial outer membrane. Biology Open 5(2):195-203. paper featured with cover image
- Sen, A., Kalvakuri, S., Bodmer, R. and Cox R. T. (2015) Clueless, a protein required for mitochondrial function, interacts with the PINK1-Parkin complex. Disease Models & Mechanisms 8(6): 577-589. paper featured with cover image
- Sen, A., Damm, V. T., and Cox R. T. (2013) Drosophila clueless is highly expressed in larval neuroblasts, affects mitochondrial localization and suppresses mitochondrial oxidative damage. PLoSONE. 8(1): e54283.
- Cox, R. T. and Spradling, A. C. (2009) clueless, a conserved Drosophila gene required for mitochondrial subcellular localization, genetically interacts with parkin. Disease Models & Mechanisms 2(9/10): 490-499.
Saibal Dey, PhD
Name: Saibal Dey, PhD
USU Department of Primary Appointment:
Biochemistry
Title:
Professor and Vice Chair
Faculty Rank:
Full Professor
Location:
Walter Reed National Military Medical Center, Bethesda, MD
Research Interests:
Membrane Transport
Multidrug Resistance
Email:
Office Phone:
(301) 295-3449
Education
Ph.D., Biochemistry, Wayne State University School of Medicine, Detroit, Michigan (1994)
M. Sc., Zoology, Specialization: Cell Biology, University of Burdwan, West Bengal, India (1985)
B. Sc., Zoology, Minor: Botany and Chemistry, University of Burdwan, West Bengal, India (1983)
M. Sc., Zoology, Specialization: Cell Biology, University of Burdwan, West Bengal, India (1985)
B. Sc., Zoology, Minor: Botany and Chemistry, University of Burdwan, West Bengal, India (1983)
Representative publications, projects, and/or deployments
- Vice Chair, Department of Biochemistry
- Dean's Fellow, Uniformed Services University School of Medicine, (2018)
- Module Director, Foundation in Medicine Module, Molecules to Military Medicine Curriculum, USU School of Medicine
- "School of Medicine Dean's Teaching Award" in Basic Science, Uniformed Services University School of Medicine (2017)
- “Hébert Impact Award” for outstanding contribution to the mission of the School, Uniformed Services University School of Medicine (2016)
- “Hébert Impact Award” for outstanding contribution to the mission of the School, Uniformed Services University School of Medicine (2015)
- “Recognition For Exemplary Support of the Molecules to Military Medicine Curriculum” Uniformed Services University School of Medicine Curriculum Reform (2011)
- “Plank Holders of the Molecules to Military Medicine Curriculum”, for contribution to curriculum reform, Dean’s Office, Uniformed Services University School of Medicine (2011)
- “Fellows Award for Research Excellence”, National Institutes of Health, Bethesda, Maryland. (1999)
- “AACR-Genetics Institute Young Investigator Award”, in the annual meeting of The American Association For Cancer Research (1998)
Bibliography
- Mandal, D., Moitra, K., Ghosh, D., Xia, D. and Dey, S. (2012) Evidence for modulatory sites at the lipid-protein interface of the human multidrug transporter P-glycoprotein. Biochemistry 51:2852-2866
- Data, S., Mazumder, S., Ghosh, D., S., Dey, S., and Bhattacharya, S. (2009) Low concentration of arsenic induce caspase-3 mediated head kidney macrophage apoptosis with JNK-p38 activation in Claris batrachas. Toxicology and Applied Pharmacology 241:329-338
- Fong, W. F., Wan, C. K., Zhu, G. Y., Chattopadhyay, A., Dey S., Zhao, Z., and Shen, X. L. (2007) Schisandrol A from Schisandra chnensis reverses P-glycoprotein-mediated multidrug resistance by affecting Pgp-substrate complexes. Planta Medica, 73: 212-220.
- Wan, C. K., Zhu, G. Y., Shen, X. L., Chattopadhyay, A., Dey, S., and Fong, W. F. (2006) Gomisin A alters substrate interaction and reverses P-glycoprotein mediated multidrug resistance in HepG2-DR cells. Biochemical Pharmacology, 72(7): 824-37
- Maki, N., and Dey, S. (2006) Biochemical and pharmacological properties of an allosteric modulator site of human P-glycoprotein. Biochemical Pharmacology 72(2): 145-155
- Maki, N., Moitra, K., Ghosh, P., and Dey, S. (2006) Allosteric modulation bypasses requirement for ATP hydrolysis in regenerating low-affinity transition state conformation of human P-glycoprotein. Journal of Biological Chemistry. 281(16): 10699-10777
- Ghosh, P., Moitra, K., Maki, N., and Dey, S. (2006) Allosteric modulation of the human P-glycoprotein involves conformational changes mimicking catalytic transition intermediates. Archives of Biochemistry and Biophysics. 450 (1): 100-112
- Maki, N., Moitra, K., Silver, C., Ghosh, P., Chattopadhyay, A. K., and Dey, S. (2006) Modulator induced interference in functional cross talk between the substrate- and the ATP- sites of human P-glycoprotein. Biochemistry 45: 2739-2751
- Maki, N., Hafkemeyer, P., and Dey, S. (2003). Allosteric modulation of human P-glycoprotein: Inhibition of transport by preventing substrate translocation and dissociation. Journal of Biological Chemistry. 278(20): 18132-18139.
- Dey, S. (2002) Biricodar (Vertex Pharmaceuticals). Current Opinion in Investigational Drugs, Vol. 3(5): 818-823.
Teresa M Dunn, PhD
Name: Teresa M Dunn, PhD
USU Department of Primary Appointment:
Biochemistry
Title:
Professor and Chair
Faculty Rank:
Full Professor
Location:
Uniformed Services University of the Health Sciences, Bethesda, MD
Education
B.S. in Biochemistry, University of Maine, Orono. 1978
Ph.D. in Biochemistry, Brandeis University, 1984
Postdoctoral Fellowship, Biological Chemistry, Johns Hopkins University School of Medicine, 1988
Ph.D. in Biochemistry, Brandeis University, 1984
Postdoctoral Fellowship, Biological Chemistry, Johns Hopkins University School of Medicine, 1988
Yuanyi Feng, MD, PhD
Name: Yuanyi Feng, MD, PhD
USU Department of Primary Appointment:
Biochemistry
Faculty Rank:
Associate Professor
Location:
Uniformed Services University of the Health Sciences, Bethesda, MD
Research Interests:
Cerebral cortical neurogenesis
Genome stability and cell differentiation; neuroprogenitor vascular interaction
Email:
Office Phone:
(301) 295-9419
Education
MD: Peking University Health Science Center, Beijing, P.R. China
PhD: Johns Hopkins University, Baltimore, Maryland
Postdoc Training: Harvard Medical School, Boston, Massachusetts
PhD: Johns Hopkins University, Baltimore, Maryland
Postdoc Training: Harvard Medical School, Boston, Massachusetts
Representative publications, projects, and/or deployments
- 2017 - Associate Professor, Department of Biochemistry and Molecular Biology, the Uniformed Services University of the Health Sciences-F. Edward Hébert School of Medicine
- 2006 - 2017 Assistant Professor, Department of Neurology, Northwestern University Feinberg School of Medicine
- 2002 - 2006 Instructor, Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School
- Current grant support: R01, NIH/NINDS, Functions of filamin in brain development and diseases (2015 - 2020)
Bibliography
- Lanctot, A.A., Guo, Y., Le Y., Edens, B.M., Nowakowski, R.S., and Feng, Y. 2017. Loss of Brap Results in Premature G1/S Phase Transition and Impeded Neural Progenitor Differentiation. Cell Rep. 20(5): 1148-60
- Houlihan, S.L.*, Lanctot, A.A.*, Guo, Y. and Feng, Y. 2016. Upregulation of neurovascular communication through filamin abrogation promotes ectopic periventricular neurogenesis. eLife,5: e17823. *These authors contributed equally
- Houlihan, S.L. & Feng, Y. 2014. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. eLife 3: e03297.
- Lanctot, A.A., Peng, C., Pawlisz, A.S., Joksimovic, M., and Feng, Y. 2013. Spatially-dependent Dynamic MAPK Modulation by the Nde1-Lis1-Brap Complex Patterns Mammalian CNS. Dev. Cell 25: 241-55 *featured article; F1000 recommend article
- Pawlisz, A.S. and Feng, Y. 2011. Three-dimensional Regulation of Radial Glial Functions by Lis1-Nde1 and Dystrophin Glycoprotein Complexes. PLoS Biol. 9(10):e1001172
- Alkuraya, F.S*, Cai, X*., Emery, C., Mochida, G. H., Al DosariM. S., FelieJ. M., Hill, R.S., Barry, B.J., Partlow, J.N., Gascon, G. G., Kentab, A., Jan, M., Shaheen, R., Feng, Y., †, and Walsh, C.A† 2011. NDE1 is mutated in extreme microcephaly, and is required for cell cycle progression through phosphorylation on its C-terminus. Am J Hum Genet. 88: 536-47 *These authors contributed equally; † Co-corresponding author
- Pawlisz, A.S., Mutch, C., Wynshaw-Boris, A., Chenn, A., Walsh, C.A. and Feng, Y. 2008. LIS1-Nde1 Dependent Neuronal Fate Control Determines Cerebral Cortical Size and Lamination. Hum Mol Genet. 17: 2441-55
- Feng, Y., Chen, M.H., Moskowitz, I., Mendonza,A.M., Vidali, L., Nakanura, F., Kwiatkowski, D.J., and Walsh, C.A. 2006. Filamin A is Required for Cell-Cell Contact in Vascular Development and Cardiac Morphogenesis. Proc Natl Acad Sci. 103: 19836-41
- Feng, Y. and Walsh, C.A. 2004. Mitotic Spindle Regulation by Nde1 (mNudE) Controls Cerebral Cortical Size. Neuron, 44: 279-93 * Featured article; F1000 recommended article
- Feng, Y., Olson, E.C., Stukenberg, P.T., Flanagan, L.A., Kirschner, M.W. and Walsh, C.A. 2000. Interactions Between LIS1 and mNudE, a Central Component of the Centrosome, are Required for CNS Lamination. Neuron 28: 665-679; * Featured article
David A Grahame, M.S., Ph.D.
Name: David A Grahame, M.S., Ph.D.
USU Department of Primary Appointment:
Biochemistry
Title:
Dr.
Faculty Rank:
Full Professor
Location:
Uniformed Services University of the Health Sciences, Bethesda, MD
Email:
Office Phone:
(301) 295-3555
Department Website:
Education
M.S. Biochemical Science 1979 University of Vermont, College of Agriculture and Life Sciences
Ph.D. Chemistry 1984 Ohio State University Department of Chemistry
Ph.D. Chemistry 1984 Ohio State University Department of Chemistry
Galina V. Petukhova, PhD
Name: Galina V. Petukhova, PhD
USU Department of Primary Appointment:
Biochemistry
Faculty Rank:
Associate Professor
Location:
Walter Reed National Military Medical Center, Bethesda, MD
Research Interests:
Homologous Recombination
Email:
Office Phone:
(301) 295-3564
Education
M.S., Dept of Biology, Moscow State University, Moscow, Russia
Ph.D., Shemyakin & Ovchinnikov Inst. of Bioorganic Chemistry, Moscow, Russia
Ph.D., Shemyakin & Ovchinnikov Inst. of Bioorganic Chemistry, Moscow, Russia
Bibliography
- Smagulova F, Brick K, Pu Y, Camerini-Otero RD, Petukhova GV. The evolutionary turnover of recombination hot spots contributes to speciation in mice (2016) Genes Dev. 30: 266-80.
- Pratto F, Brick K, Khil P, Smagulova F, Petukhova GV, Camerini-Otero RD. Recombination initiation maps of individual human genomes (2014) Science 346:1256442.
- Brick, K., Smagulova, F., Khil, P., Camerini-Otero, R.D., and Petukhova, G.V. Genetic recombination is directed away from functional genomic elements in mice (2012) Nature 485:642-645.
- Smagulova, F., Gregoretti, I.V., Brick, K., Khil, P., Camerini-Otero, R.D., and Petukhova, G.V. Genome-wide analysis reveals novel molecular features of mouse recombination hotspots (2011) Nature 472:375-378.
Mark A Roseman, Ph.D.
Name: Mark A Roseman, Ph.D.
USU Department of Primary Appointment:
Biochemistry
Faculty Rank:
Associate Professor
Location:
Uniformed Services University of the Health Sciences, Bethesda, MD
Email:
Office Phone:
(301) 295-3570
Education
B.S., Sept. 1961 - May 1965 University of Michigan, Ann Arbor, Michigan
Major: Cell Biology (combined chemistry and
biology program)
Ph.D., June 1965 - Jan. 1971 Michigan State University, East Lansing, Michigan.
Major: Biochemistry; Minor: Chemistry.
Thesis title: "A Model System Analysis of the Mechanism of
2-keto-3-deoxy-6-phosphogluconic Acid Aldolase".
Major: Cell Biology (combined chemistry and
biology program)
Ph.D., June 1965 - Jan. 1971 Michigan State University, East Lansing, Michigan.
Major: Biochemistry; Minor: Chemistry.
Thesis title: "A Model System Analysis of the Mechanism of
2-keto-3-deoxy-6-phosphogluconic Acid Aldolase".
Biography
Born: October 30, 1944, Brooklyn, New York.
Undergraduate: University of Michigan, 1961-5
Married: 1965-present, to Jo Ellen Roseman; 2 sons, 4 grandchildren
Hobby: Scuba
Undergraduate: University of Michigan, 1961-5
Married: 1965-present, to Jo Ellen Roseman; 2 sons, 4 grandchildren
Hobby: Scuba
Representative publications, projects, and/or deployments
- June 1973 - June 1979 Research Associate, Department of Biochemistry, University of Virginia. In the laboratory of Dr. T. E. Thompson, engaged in physical and organic chemical studies on artificial membranes.
- . Jan. 1971 - June 1973 N.I.H. Postdoctoral Fellow, Department of Biochemistry, Brandeis University. In the laboratory of Dr. W. P. Jencks. Studies on the interaction of urea and other denaturants with polar molecules in water.
- 1994-2008 Course Director, Medical Biochemistry, Uniformed Services University, Department of Biochemistry
- 2008- present. Co-course Director for biochemistry teaching in the integrated curriculum at Uniformed Services University
- May 15, 2015, Gold Medal in Columbia Triathalon Relay (Swim leg).
Bibliography
- Roseman, M. A. and Jencks, W. P., "Interaction of Urea and Other Polar Compounds in Water", J. Am. Chem. Soc. 97, 631-640 (1975).
- Roseman, M. A., Litman, B. J. and Thompson, T. E., "Transbilayer Exchange of Phosphatidylethanolamine for Phosphatidylcholine and Amidino- Phosphatidylethanolamine in Single Walled Bilayer Vesicles", Biochemistry 14, 4826-4830 (1975).
- Roseman, M. A., Holloway, P. W. Calabro, M. A. and Thompson, T. E., "The Exchange of Cytochrome b5 Between Phospholipid Vesicles", J. Biol. Chem. 252, 4842-4849 (1977).
- Roseman, M. A. and Thompson, T. E., "Mechanism of the Spontaneous Transfer of Phospholipids between Bilayers", Biochemistry 19, 439-444 (1980).
- Greenhut, Susan F. and Roseman, Mark A., "Cytochrome b5 Induced Flip- Flop of Phospholipids in Sonicated Vesicles", Biochemistry 24, 1252-1260 (1985)..
- Greenhut, Susan F. and Roseman, Mark A., "Distribution of Cytochrome b5 Between Sonicated Phospholipid Vesicles of Different Size", J. Biol. Chem. 260, 5883-5886 (1985).
- Roseman, M. A., "Hydrophilicity of Polar Amino Acid Side Chains is Markedly Reduced by Flanking Peptide Bonds", J. Mol. Biol. 200, 513-522 (1988)
- Roseman, M. A., "Hydrophobicity of the Peptide C-O---H-N Hydrogen Bonded Group", J. Mol. Biol. 201, 621-623 (1988)
- Grant, Jr., E., Beeler, T. J., Taylor, K. M. P., Gable, K., and Roseman, M. A., "Mechanism of Magainin 2a Induced Permeabilization of Phospholipid Vesicles," Biochemistry 31, 9912-9918 (1992).
- Taylor, K. M. P., and Roseman, M. A., "The Effect of Cholesterol, Fatty Acyl Chain Composition, and Bilayer Curvature on the Interaction of Cytochrome b5 with Liposomes of Phosphatidylcholines," Biochemistry 34, 3841-50 (1995).
Jeremy Rotty, Ph.D.
Name: Jeremy Rotty, Ph.D.
USU Department of Primary Appointment:
Biochemistry
Faculty Rank:
Assistant Professor
Location:
Uniformed Services University of the Health Sciences, Bethesda, MD
Research Interests:
Cell Migration
Immunology
Email:
Office Phone:
(301) 295-3550
Education
Berea College
B.A. in Biology, 2005
The Johns Hopkins School of Medicine
Ph.D. BCMB program, 2011
The University of North Carolina at Chapel Hill
Postdoctoral Fellow, Dept. of Cell Biology and Physiology, 2017
B.A. in Biology, 2005
The Johns Hopkins School of Medicine
Ph.D. BCMB program, 2011
The University of North Carolina at Chapel Hill
Postdoctoral Fellow, Dept. of Cell Biology and Physiology, 2017
Bibliography
- Rotty, JD., Brighton, HE., Asokan, SB., Cheng, N., Ting, JP., and Bear, JE. (2017) Arp2/3 complex is required for macrophage integrin functions but is dispensable for FcR phagocytosis and in vivo motility. Dev Cell. 2017 August 9; 42(5): 498-513.
- Rotty, JD and Bear, JE. (2015) Competition and collaboration between different actin assembly pathways allows for homeostatic control of the actin cytoskeleton. BioArchitecture. Review. 2015 Aug 3; 5(1-2):27-34. Epub 2015 Oct. 2.
- Rotty, JD., Wu, C., Haynes, EM., Suarez, C., Winkelman, JD., Johnson, HE., Haugh. JM., Kovar, DR., and Bear, JE. (2015) Profilin-1 serves as a gatekeeper for actin assembly by Arp2/3-dependent and –independent pathways. Dev Cell. 2015 Jan 12;32(1):54-67
- Rotty, JD., Wu, C., and Bear, JE. (2013) New insights into the regulation and cellular functions of the Arp2/3 complex. Nat. Rev. Mol. Cell Biol. Review. 14(1):7-12
- Rotty, JD. and Coulombe, PA. (2012) A wound-induced keratin inhibits Src activity during keratinocyte migration and tissue repair. J. Cell Biol. 197(3): 381-9
Prasanna Satpute-Krishnan, Ph.D.
Name: Prasanna Satpute-Krishnan, Ph.D.
USU Department of Primary Appointment:
Biochemistry
Title:
Dr.
Faculty Rank:
Assistant Professor
Location:
Uniformed Services University of the Health Sciences, Bethesda, MD
Research Interests:
Cell Biology
Protein Trafficking and Quality Control
Email:
Office Phone:
(301) 295-2845
Department Website:
Education
Postdoctoral Training: Jennifer Lippincott-Schwartz Lab at National Institutes of Health, Bethesda, MD
https://science.nichd.nih.gov/confluence/display/sob/Home
Graduate Education: Ph.D. in Molecular Biology, Cell Biology and Biochemistry at Brown University, Providence, RI
Thesis title: "Mechanistic Insights Into Prion Propagation by Monitoring Protein Dynamics In Vivo"
https://www.brown.edu/academics/biology/molecular-cell-biochemistry/graduate/mcb-graduate-program
https://science.nichd.nih.gov/confluence/display/sob/Home
Graduate Education: Ph.D. in Molecular Biology, Cell Biology and Biochemistry at Brown University, Providence, RI
Thesis title: "Mechanistic Insights Into Prion Propagation by Monitoring Protein Dynamics In Vivo"
https://www.brown.edu/academics/biology/molecular-cell-biochemistry/graduate/mcb-graduate-program
Representative publications, projects, and/or deployments
- 2014 Bernfield Award for Excellence in Research, American Society for Cell Biology
- 2007 Joukowsky Award for the top thesis in the life sciences, Brown University
Bibliography
- Sengupta P, Satpute-Krishnan P, Seo A, Burnette DT, Patterson G, and Lippincott-Schwartz J, ER trapping reveals Golgi enzymes continually revisit the ER through a recycling pathway that controls Golgi organization. Proc Natl Acad Sci USA 2015 Dec 8; 112(49): E6752-61
- Satpute-Krishnan P, Ajinkya M, Bhat S, Hegde RS, Lippincott-Schwartz J. ER stressinduced clearance of misfolded GPI-anchored proteins via the secretory pathway. Cell. 2014 July 31; 158(3):522-33**
- Hailey DW, Rambold AS, Satpute-Krishnan P, Mitra K, Sougrat R, Kim PK, Lippincott- Schwartz J. Mitochondria supply membranes for autophagosome biogenesis during starvation Cell. 2010 May 14; 141(4):656-67.
- Satpute-Krishnan P, Langseth SX, Serio TR. Hsp104-dependent remodeling of prion complexes mediates protein-only inheritance. PLoS Biol. 2007 Jan; 5(2):e24.
- Satpute-Krishnan P, Serio TR. Prion protein remodelling confers an immediate phenotypic switch. Nature. 2005 Sep 8; 437(7056):262-5.
Tharun Sundaresan, Ph.D.
Name: Tharun Sundaresan, Ph.D.
USU Department of Primary Appointment:
Biochemistry
Title:
Associate Professor
Faculty Rank:
Associate Professor
Location:
Uniformed Services University of the Health Sciences, Bethesda, MD
Research Interests:
Mechanism of mRNA decay
Molecular basis of cancer
Email:
Office Phone:
(301) 295-9423
Department Website:
Education
Ph.D. (Life Sciences) - Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India (Affiliated to Jawaharlal Nehru University, New Delhi, India).
Representative publications, projects, and/or deployments
- Tharun, S and Parker, R. 1995. Mechanisms of mRNA Turnover in Eukaryotic Cells. In Modern Biology Series vol:17 (mRNA metabolism and post-transcriptional gene regulation), p181-199. Ed. Harford, J.B., and Morris, D.R. John Wiley Publishers.
- Tharun, S and Parker, R. 1999. Turnover of mRNA in eukaryotic cells. In Comprehensive natural products chemistry vol:6 (Prebiotic chemistry, molecular fossils, nucleosides and RNA), p205-216. Ed. Barton, S.D and Nakanishi, K. Pergamon Publishers.
- Tharun, S. 2009. Lsm1-7-Pat1 complex: A link between 3’ and 5’-ends in mRNA decay? RNA Biology, 6(3):228-232.
- Tharun, S. 2009. Roles of eukaryotic Lsm proteins in the regulation of mRNA function. International Review of Cell & Molecular Biology, 272:149-189.
- HHMI Fellowship
- American Cancer Society IRG grant
- NIH RO1 Research project grant
- USUHS Exploratory grant
- Faculty (secondary appointment), Molecular Cell Biology Graduate Program, Uniformed Services University of the Health Sciences.
- Course Director, Graduate Biochemistry course, Uniformed Services University of the Health Sciences.
Bibliography
- Chowdhury, A, Swathi Kalurupalle, S and Tharun, S*. 2016. Mutagenic analysis of the C-terminal domain of Lsm1. PLoS ONE, 11: e0158876.
- Jungfleisch, J., Chowdhury, A, Alves-Rodrigues, I, Tharun, S* and Juana Díez. 2015. The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by 3 differential mechanisms. RNA, 21:1469–1479.
- Chowdhury, A, Kalurupalle, S, and Tharun, S*. 2014. Pat1 contributes to the RNA binding activity of the Lsm1-7-Pat1 complex. RNA, 20:1465–1475.
- Chowdhury, A, Raju, KK, Kalurupalle, S and Tharun, S*. 2012. Both Sm-domain and C-terminal extension of Lsm1 are important for the RNA-binding activity of the Lsm1–7–Pat1 complex. RNA, 18:936–944.
- Chowdhury, A, and Tharun, S*. 2009. Activation of decapping involves binding of the mRNA and facilitation of the post-binding steps by the Lsm1-7-Pat1 complex. RNA, 15:1837–1848.
- Tharun, S. 2008. Purification and analysis of the decapping activator Lsm1p-7p-Pat1p complex from yeast. Methods in Enzymology, 448:41-55.
- Chowdhury, A, and Tharun, S*. 2008. lsm1 mutations impairing the ability of the Lsm1p-7p-Pat1p complex to preferentially bind to oligoadenylated RNA affect mRNA decay in vivo. RNA, 14:2149-2158.
- Chowdhury, A, Mukhopadhyay, J. and Tharun, S*. 2007. The decapping activator Lsm1p-7p-Pat1p complex has the intrinsic ability to distinguish between oligoadenylated and polyadenylated RNAs. RNA, 13:998-1016.
- Tharun S*, Muhlrad D, Chowdhury A and Parker R. 2005. Mutations in the Saccharomyces cerevisiae LSM1 gene that affect mRNA decapping and 3' end protection. Genetics, 170:33-46. *Corresponding author
- Tharun, S* and Parker R. 2001. Targeting an mRNA for decapping: Displacement of translation factors and association of the Lsm1p-7p complex on deadenylated yeast mRNAs. Molecular Cell, 8:1075-1083. *Corresponding author
Shuishu Wang, Ph.D.
Name: Shuishu Wang, Ph.D.
USU Department of Primary Appointment:
Biochemistry
Faculty Rank:
Assistant Professor
Location:
Uniformed Services University of the Health Sciences, Bethesda, MD
Email:
Office Phone:
(301) 295-3418
Education
Ph.D., Purdue University
Postdoctoral, UCLA
Postdoctoral, UCLA
Representative publications, projects, and/or deployments
- 2008 – pres. Assistant Professor, Department of Biochemistry and Molecular Biology, F. Edward Hebert School of Medicine, Uniformed Services University, Bethesda, Maryland.
- 2004 – 2008 Assistant Member, Public Health Research Institute, Newark, New Jersey.
- 2006 – 2008 Assistant Professor, Department of Biochemistry and Molecular Biology, New Jersey Medical School, Newark, New Jersey.
- National Institute of General Medical Sciences, R01GM079185, Structural and functional studies of a PhoP-PhoR two-component system, 7/20/2007 – 5/31/2012, no cost extension to 5/31/2015, total costs for the entire grant period $1,400,490, role PI.
- USUHS intramural exploratory grant, Bio-71-3018, Mechanisms of a PhoPR signaling system from Mycobacterium tuberculosis, 2/2015 – 9/2017, $150,000 direct costs + $71,765 indirect costs, role PI.
- USUHS intramural bridge funding award. Phosphorylation and signaling mechanism of the PhoPR two-component system, 4/2017 – 4/2018, $50,000 direct costs + $25,000 indirect costs, role PI.
Bibliography
- 1. L. Wang, D. Xing, A. Le Van, A.E. Jerse, and S. Wang. Structure-based design of ferritin-nanoparticle immunogens displaying antigenic loops of Neisseria gonorrhoeae. FEBS Open Bio (2017) 7, 1196-1207.
- 2. D. Xing, M.B. Ryndak, L.Wang, I. Kolesnikova, I. Smith, and S. Wang. Asymmetric structure of the dimerization domain of PhoR, a sensor kinase important for the virulence of Mycobacterium tuberculosis. ACS Omega (2017) 2, 3509-17.
- 3. L. Wang, M. Xu, N. Southall, W. Zheng, and S. Wang. A High-Throughput Assay for Developing Inhibitors of PhoP, A Virulence Factor of Mycobacterium tuberculosis. Comb Chem High Throughput Screen. (2016) 19, 855-864.
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Xin Xiang, Ph. D.
Name: Xin Xiang, Ph. D.
USU Department of Primary Appointment:
Biochemistry
Title:
Professor
Faculty Rank:
Full Professor
Location:
Uniformed Services University of the Health Sciences, Bethesda, MD
Research Interests:
Cell biology, intracellular transport, motor proteins
Genetics, fungi, Aspergillus nidulans
Email:
Office Phone:
(301) 295-0000
Education
B.S. & M.S. Department of Biology, Peking University, Beijing, P. R. China
Ph.D. Department of Biochemistry, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey. Advisor: Dr. Kiran Chada.
Post-doctoral research: Department of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey. Advisor: Dr. Ron Morris.
Ph.D. Department of Biochemistry, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey. Advisor: Dr. Kiran Chada.
Post-doctoral research: Department of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey. Advisor: Dr. Ron Morris.
Biography
2012- Professor, Department of Biochemistry and Molecular Biology, the Uniformed Services University of the Health Sciences-F. Edward Hébert School of Medicine
2004-2012 Associate Professor, Department of Biochemistry and Molecular Biology, the Uniformed Services University of the Health Sciences-F. Edward Hébert School of Medicine
1999-2004 Assistant professor, Department of Biochemistry and Molecular Biology, the Uniformed Services University of the Health Sciences-F. Edward Hébert School of Medicine
2004-2012 Associate Professor, Department of Biochemistry and Molecular Biology, the Uniformed Services University of the Health Sciences-F. Edward Hébert School of Medicine
1999-2004 Assistant professor, Department of Biochemistry and Molecular Biology, the Uniformed Services University of the Health Sciences-F. Edward Hébert School of Medicine
Representative publications, projects, and/or deployments
- Xiang, X. (2018) Insights into Cytoplasmic Dynein Function and Regulation from Fungal Genetics (book chapter), in: S. M. King (Ed.) Dyneins: Structure, Biology and Disease: The Biology of Dynein Motors, vol. 1, Academic Press, Elsevier, 2018, pp. 470-501.
- Xiang, X. (2017) Nuclear Movement in fungi (review). Seminars in Cell and Developmental Biology: in the special issue "Nuclear positioning" (Guest Editor: B. Cadot), in press, https://doi.org/10.1016/j.semcdb.2017.10.024
- Xiang, X, Qiu, R., Yao, X., Arst, H.N. Jr, Peñalva, M.A. and Zhang, J. (2015) Cytoplasmic dynein and early endosome transport (review). Cellular and molecular life sciences 72, 3267-3280. PMCID: PMC4534323
- Xiang, X. (2012) Nuclear positioning: dynein needed for microtubule shrinkage-coupled movement. Curr. Biol. 22 (12), R496-R499.
- Xiang, X. and Oakley, B. R. (2010). The Cytoskeleton in Filamentous Fungi (book chapter). In: Cellular and Molecular Biology of Filamentous Fungi (Edited by K. Borkovich and D. Ebbole), ASM Press. p209-223.
- Xiang, X. (2006) A +TIP for a smooth trip. (minireview) J. Cell Biol. 172, 651-654.
- Wu, X., Xiang, X., Hammer, J. (2006) Motor proteins at microtubule plus end. (review), Trends in Cell Biology 16,135-143.
- Current grant support: RO1, NIH/NIGMS "Regulation of cytoplasmic dynein in vivo" (2017-2021).
- USUHS Awards (2017): Henry Wu award for excellence in the basic sciences; Medical School Dean's Impact award recognizing excellence in research.
Bibliography
Biosketch Link:
- Xiang, X., Benson, K. and Chada, K. (1990) Mini-mouse: Disruption of the pygmy locus in a transgenic insertional mutant. Science 247, 967-969.
- Xiang, X., Beckwith, S. M. and Morris, N. R. (1994) Cytoplasmic dynein is involved in nuclear migration in Aspergillus nidulans. Proc. Natl. Acad. Sci. USA 91, 2100-2104.
- Xiang, X., Osmani, A. H., Osmani, S. A., Xin, M. and Morris, N. R. (1995) NudF, a nuclear migration gene in Aspergillus nidulans, is similar to the human LIS-1 gene required for neuronal migration. Mol. Biol. Cell 6, 297-310.
- Han, G., Liu, B., Zhang, J., Zuo W., Morris, N. R. and Xiang, X. (2001) The Aspergillus cytoplasmic dynein heavy chain and NUDF localize at the microtubule ends and affect microtubule dynamics. Curr. Biol. 11, 719-724.
- Zhang, J., Li, S., Fischer, R. and Xiang, X. (2003) Accumulation of cytoplasmic dynein and dynactin at microtubule plus ends in Aspergillus nidulans is kinesin dependent. Mol. Biol. Cell 14, 1479-1488.
- Zhang, J., Yao, X., Fischer, L. Abenza J. F., Peñalva. M. A., Xiang, X. (2011) The p25 subunit of the dynactin complex is required for dynein-early endosome interaction. J. Cell Biol. 193, 1245-1255. PMCID:PMC3216330
- Qiu, R., Zhang, J., Xiang, X. (2013) Identification of a novel site in the tail of dynein heavy chain important for dynein function in vivo. J. Biol. Chem. 288, 2271-2280.
- Zhang, J., Qiu, R., Arst, H.N. Jr, Peñalva, M.A. and Xiang, X. (2014) HookA is a novel dynein-early endosome linker critical for cargo movement in vivo. J. Cell Biol., 204, 1009-1026 PMCID: PMC3998793. (highlighted by JCB In Focus and by Nat. Rev. Mol. Cell Biol in Res. Highlights).
- Yao, X., Wang, X. and Xiang, X. (2014) FHIP and FTS proteins are critical for dynein-mediated transport of early endosomes in Aspergillus. Mol. Biol. Cell. 25, 2181-2189 (highlighted by MBoC). PMCID: PMC4091831
- Yao, X., Arst, H, Wang, X. and Xiang, X. (2015) Discovery of a vezatin-like protein for dynein-mediated early endosome transport. Mol. Biol. Cell. 26, 3816-3827 (highlighted by MBoC). PMCID: PMC4626066
