The Translational Imaging Facility (TIF) provides advanced, state-of-the-art neurological imaging techniques to support in vivo and ex vivo traumatic brain injury (TBI) research. The TIF helps improve our understanding of TBI by bridging the same imaging techniques between translational and human studies. It collaborates closely with other CNRM translational research initiatives by providing imaging services for the Preclinical Studies Core, the Translational Therapeutics Core, and the Optimizing Ferret TBI Experiment. Its facilities include three laboratories with imaging capabilities to include:
- A Siemens Inveon small animal Positron Emission Tomography, Single Photon Emission Computed Tomography and Computed Tomography (PET/SPECT/CT) scanner. This docked system provides a 12 cm bore with resolution of 1500 microns for PET, 1000 microns for SPECT, and 40 microns for CT. Image reconstruction includes static and dynamic framing with several reconstruction options.
- A Bruker BioSpec 7T/20 Magnetic Resonance Imaging (MRI) scanner. The scanner’s gradient insert provides a bore size of 11.4 cm with a 660 mT/m gradient strength. The lab offers phased array receive coil capabilities for both rat and mouse, 20, 35 and 72 mm transmit/receive coils, and X-nuclei transmit capabilities. It supports Bruker Paravision 5 and 6 software. Typical in plane in vivo resolution of 100-200 microns and ex vivo resolution of 50 microns isotropic.
- A Bruker Xtreme II provides fluorescent, bioluminescent, optical, and isotopic imaging; a Bruker Skyscan 1278 provides high throughput Computed Tomography for in vivo whole body resolution of 50 microns in only a few minutes; and a Bruker Skyscan 1172 provides specimen computed tomography capable of 1 micron resolution.
The TIF also helps with protocol development, study design, and data acquisition, analysis, and interpretation. It maintains four image processing workstations with various software packages, such as:
- The Siemens image processing software (5 seats), which includes co-registration, 3D visualization and analysis, and pharmacokinetic modeling
- VivoQuantTM (InVicro, LLC) software, which includes manual and automated co-registration for PET/CT/MRI, automated reference region normalization, rat and mouse brain atlas analysis, and region of interest tools
- Other non-commercial software packages including PMOD, FIJI/ImageJ, Tortoise, DTI-TK, FSL, BrainSuite, SPM, MatLab, AFNI, SMART, MIPAV, and DCE@urLAB
For more information about the TIF, please email CNRMTIF@usuhs.edu.