John Dumler, M.D.

John Dumler, M.D.

Name: John Dumler, M.D.

Department of Primary Appointment: Pathology
Position: USU Faculty
Title: Professor & Chair

Email: john.dumler@usuhs.edu (link sends e-mail)
Office Phone: (301) 295-3450
Room: B3152

Profile

 

Current Research Interests:

 

 

Vector- and tick-borne diseases and pathogenic agents

 

  • Studies of the basic biology to clinical aspects of vector-borne infections of humans and animals.
  • Focus on tick-borne infections, especially those caused by obligate intracellular bacteria in the order Rickettsiales.

 

 

 

Anaplasma phagocytophilum AnkA and epigenetic eukaryotic gene regulation

 

  • Bacterial cellular and molecular mechanisms of adherence and entry
  • Mechanisms by which the bacteria manipulate host cell transcriptional programs
  • AnkA, a protein injected into host cells that alters chromatin and nuclear structure and influences eukaryotic gene transcription
  • Genomics

 

 

 

Bacterial infections and immunopathology mediated by NKT and cytotoxic T cells

 

  • A. phagocytophilum immunopathology related to NKT, NK, and CD8 T cells immune responses.
  • Stat1 signaling and its role in immunopathology with A. phagocytophilum infection.

 

 

 

Bacterial coinfections and their synergistic mechanisms in pathology

 

  • Interactions between Lyme disease and HGA clinical presentation and severity.
  • The role of matrix metalloproteases and the fibrinolytic system in transendothelial cell dissemination

 

 

 

Vascular permeability, pathogen entry, invasion and dissemination

 

  • Transmigration of endothelial cell barriers by Borrelia, Rickettsia, Ehrlichia and other pathogens
  • The role of intracellular calcium concentrations, divalent cation chelators, phospholipase C inhibitors and signaling through G protein coupled receptors

 

 

 

Clinical investigation of febrile disease etiology in under-resourced regions

 

  • Specific microbial etiologies of febrile diseases in regions where resources are limited.
  • Objective diagnostic approaches, misdiagnosis, inappropriate treatment and the consequent negative outcomes.
  • How much fever in such regions is the result of undiagnosed rickettsial disease?
  • Clinical studies and trials to test new diagnostic tests, new therapies and vaccines.
  • High-throughput molecular diagnostic methods in Malaysia, Sri Lanka, and Nicaragua.

 

 

 

Selected Publications since 2014

 

 

 

1.      Sinclair SHG, Garcia-Garcia JC, Dumler JS. Bioinformatic and mass spectrometry identification of Anaplasma phagocytophilum proteins translocated into host cell nuclei. Front Microbiol 2015 6:55. PMC4319465.

 

2.      Bakken JS, Dumler JS. Human Granulocytic Anaplasmosis. Infect Dis Clin North Am. 2015; 29:341-355. PMC4441757

 

3.      Li H, Zheng YC, Ma L, Jia N, Jiang BG, Jiang RR, Huo QB, Wang YW, Liu HB, Chu YL, Song YD, Yao NN, Sun T, Zeng FY, Dumler JS, Jiang JF, Cao WC. Human infection with a novel tick-borne Anaplasma species in China: a surveillance study. Lancet Infect Dis 2015 Mar 27. pii: S1473-3099(15)70051-4. PMID: 25833289

 

4.      Chikeka I, Dumler JS. Neglected Bacterial Zoonoses. Themed Review in Clin Microbiol Infect Dis 2015; 21:404-415. PMC4466158

 

5.      Rennoll-Bankert KE, Garcia-Garcia JC, Sinclair SH, Dumler JS. Chromatin bound bacterial effector AnkA recruits HDAC1 and modifies host gene expression. Cell Microbiol 2015; 17:1640-52. PMID: 25996657.

 

6.      Sinclair SHG, Yegnasubramanian S, Dumler JS. Global DNA methylation changes and differential gene expression in Anaplasma phagocytophilum-infected human neutrophils. Clin Epigenetics 2015; 7:77. PMC4518890

 

7.      André MR, Dumler JS, Herrera HM, Gonçalves LR, de Sousa KCM, Scorpio DG, Alexandre de Santis ACG, Domingos IH, Carvalho de Macedo G, Zacarias Machado R. Assessment of a quantitative 5’ nuclease real-time polymerase chain reaction using the nicotinamide adenine dinucleotide dehydrogenase gamma subunit (nuoG) for Bartonella species in domiciled and stray cats in Brazil. J Fel Med Surg 2015 [in press].

 

8.      Dittrich S, Phuklia W, Turner GDH, Rattanavong S, Dumler JS, Paris DH, Newton PN. Sennetsu as a neglected fish-borne cause of fever in South-East Asia. PLoS Negl Trop Dis. 2015; 9:e0003908. doi:10.1371/journal.pntd.0003908. PMC4497638.

 

9.      Huang X, Zhang X, Wang F, Wei H, Ma H, Sui M, Lu J, Wang H, Dumler JS, Sheng G, Xu B. Clinical efficacy of therapy with recombinant human interferon α1b in hand, foot, and mouth disease with Enterovirus 71 infection. PLoS One. 2016; 11:e0148907. PMC4755579.

 

10.  Wang X, Shaw DK, Sakhon OS, Snyder GA, Sundberg EJ, Santambrogio L, Sutterwala FS, Dumler JS, Shirey KA, Perkins DJ, Richard K, Chagas AC, Calvo E, Kopecký J, Kotsyfakis M, Pedra JH. The tick protein sialostatin L2 binds to annexin A2 and inhibits NLRC4-mediated inflammasome activation. Infect Immun. 2016. [Epub ahead of print] PMID: 27045038.

 

11.  Nikolskaia O Thekisoe O, Dumler JS, Grab DJ. Lamp for detection of the 5.8s rRNA-internal transcribed spacer 2 gene found in Trypanosoma brucei gambiense. Am J Trop Med Hyg [in press].

 

12.  Chikeka I, Matute AJ, Dumler JS, Woods CW, Mayorga O, Reller ME. Use of peptide ELISA followed by IFA to document Ehrlichia chaffeensis as a cause of febrile illness in Nicaragua. J Clin Microbiol 2016; 54:1581-1585.PMC4879277.

 

13.  Wurtz N, Dasch G, Papa A, Reusken C, Van Doornum GJ, Hukic M, Rollin PE, Di caro A, Leparc-Goffard I, Leroy EM, Landini MP, Sekeyova Z, Dumler JS, Tolou H, Bădescu D, Meyer H, Marti NB, Calistri P, Parolin C, Palù G, Christova I, Heinzen RA, Reynes JM, Maurin M, Raoult D. Survey of laboratory-acquired infections in the world in biosafety level 3 and 4 laboratories. Clin Microbiol Infect Dis [in press].

 

14.  Paris DH, Dumler JS. State of the art of diagnosis of rickettsial diseases: the use of blood specimens for diagnosis of scrub typhus, spotted fever group rickettsiosis, and murine typhus. Curr Op Infect Dis [in press].

 

15.  Nikolskaia OV, Thekisoe OM, Dumler JS, Grab DJ. Loop-mediated isothermal amplification for detection of the 5.8S ribosomal ribonucleic acid internal transcribed spacer 2 gene found in Trypanosoma brucei gambiense. Am J Trop Med Hyg. 2016 [in press].